Activated platelets in carotid artery thrombosis in mice can be selectively targeted with a radiolabeled single-chain antibody.
Identifieur interne : 001608 ( Main/Exploration ); précédent : 001607; suivant : 001609Activated platelets in carotid artery thrombosis in mice can be selectively targeted with a radiolabeled single-chain antibody.
Auteurs : RBID : pubmed:21479193English descriptors
- KwdEn :
- Animals, Autoradiography, Carotid Artery Thrombosis (physiopathology), Carotid Artery Thrombosis (radiography), Carotid Artery Thrombosis (radionuclide imaging), Epitopes (immunology), Indium Radioisotopes, Isotope Labeling (methods), Mice, Platelet Activation (physiology), Protein Binding, Single-Chain Antibodies (diagnostic use), Tomography, Emission-Computed, Single-Photon, Tomography, X-Ray Computed.
- MESH :
- chemical , diagnostic use : Single-Chain Antibodies.
- chemical , immunology : Epitopes.
- methods : Isotope Labeling.
- physiology : Platelet Activation.
- physiopathology : Carotid Artery Thrombosis.
- radiography : Carotid Artery Thrombosis.
- radionuclide imaging : Carotid Artery Thrombosis.
- Animals, Autoradiography, Indium Radioisotopes, Mice, Protein Binding, Tomography, Emission-Computed, Single-Photon, Tomography, X-Ray Computed.
Abstract
Activated platelets can be found on the surface of inflamed, rupture-prone and ruptured plaques as well as in intravascular thrombosis. They are key players in thrombosis and atherosclerosis. In this study we describe the construction of a radiolabeled single-chain antibody targeting the LIBS-epitope of activated platelets to selectively depict platelet activation and wall-adherent non-occlusive thrombosis in a mouse model with nuclear imaging using in vitro and ex vivo autoradiography as well as small animal SPECT-CT for in vivo analysis.
DOI: 10.1371/journal.pone.0018446
PubMed: 21479193
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Activated platelets in carotid artery thrombosis in mice can be selectively targeted with a radiolabeled single-chain antibody.</title><author><name sortKey="Heidt, Timo" uniqKey="Heidt T">Timo Heidt</name><affiliation wicri:level="3"><nlm:affiliation>Department of Cardiology and Angiology, University of Freiburg, Freiburg, Germany. timo.heidt@uniklinik-freiburg.de</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Cardiology and Angiology, University of Freiburg, Freiburg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Fribourg-en-Brisgau</region><settlement type="city">Fribourg-en-Brisgau</settlement></placeName></affiliation></author><author><name sortKey="Deininger, Friederike" uniqKey="Deininger F">Friederike Deininger</name></author><author><name sortKey="Peter, Karlheinz" uniqKey="Peter K">Karlheinz Peter</name></author><author><name sortKey="Goldschmidt, J Rgen" uniqKey="Goldschmidt J">Jürgen Goldschmidt</name></author><author><name sortKey="Pethe, Annette" uniqKey="Pethe A">Annette Pethe</name></author><author><name sortKey="Hagemeyer, Christoph E" uniqKey="Hagemeyer C">Christoph E Hagemeyer</name></author><author><name sortKey="Neudorfer, Irene" uniqKey="Neudorfer I">Irene Neudorfer</name></author><author><name sortKey="Zirlik, Andreas" uniqKey="Zirlik A">Andreas Zirlik</name></author><author><name sortKey="Weber, Wolfgang A" uniqKey="Weber W">Wolfgang A Weber</name></author><author><name sortKey="Bode, Christoph" uniqKey="Bode C">Christoph Bode</name></author><author><name sortKey="Meyer, Philipp T" uniqKey="Meyer P">Philipp T Meyer</name></author><author><name sortKey="Behe, Martin" uniqKey="Behe M">Martin Behe</name></author><author><name sortKey="Von Zur M Hlen, Constantin" uniqKey="Von Zur M Hlen C">Constantin von Zur Mühlen</name></author></titleStmt><publicationStmt><date when="2011">2011</date><idno type="doi">10.1371/journal.pone.0018446</idno><idno type="RBID">pubmed:21479193</idno><idno type="pmid">21479193</idno><idno type="wicri:Area/Main/Corpus">001437</idno><idno type="wicri:Area/Main/Curation">001437</idno><idno type="wicri:Area/Main/Exploration">001608</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Autoradiography</term><term>Carotid Artery Thrombosis (physiopathology)</term><term>Carotid Artery Thrombosis (radiography)</term><term>Carotid Artery Thrombosis (radionuclide imaging)</term><term>Epitopes (immunology)</term><term>Indium Radioisotopes</term><term>Isotope Labeling (methods)</term><term>Mice</term><term>Platelet Activation (physiology)</term><term>Protein Binding</term><term>Single-Chain Antibodies (diagnostic use)</term><term>Tomography, Emission-Computed, Single-Photon</term><term>Tomography, X-Ray Computed</term></keywords><keywords scheme="MESH" type="chemical" qualifier="diagnostic use" xml:lang="en"><term>Single-Chain Antibodies</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Epitopes</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Isotope Labeling</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Platelet Activation</term></keywords><keywords scheme="MESH" qualifier="physiopathology" xml:lang="en"><term>Carotid Artery Thrombosis</term></keywords><keywords scheme="MESH" qualifier="radiography" xml:lang="en"><term>Carotid Artery Thrombosis</term></keywords><keywords scheme="MESH" qualifier="radionuclide imaging" xml:lang="en"><term>Carotid Artery Thrombosis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Autoradiography</term><term>Indium Radioisotopes</term><term>Mice</term><term>Protein Binding</term><term>Tomography, Emission-Computed, Single-Photon</term><term>Tomography, X-Ray Computed</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Activated platelets can be found on the surface of inflamed, rupture-prone and ruptured plaques as well as in intravascular thrombosis. They are key players in thrombosis and atherosclerosis. In this study we describe the construction of a radiolabeled single-chain antibody targeting the LIBS-epitope of activated platelets to selectively depict platelet activation and wall-adherent non-occlusive thrombosis in a mouse model with nuclear imaging using in vitro and ex vivo autoradiography as well as small animal SPECT-CT for in vivo analysis.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">21479193</PMID><DateCreated><Year>2011</Year><Month>04</Month><Day>11</Day></DateCreated><DateCompleted><Year>2011</Year><Month>07</Month><Day>26</Day></DateCompleted><DateRevised><Year>2013</Year><Month>10</Month><Day>18</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>6</Volume><Issue>3</Issue><PubDate><Year>2011</Year></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS ONE</ISOAbbreviation></Journal><ArticleTitle>Activated platelets in carotid artery thrombosis in mice can be selectively targeted with a radiolabeled single-chain antibody.</ArticleTitle><Pagination><MedlinePgn>e18446</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0018446</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Activated platelets can be found on the surface of inflamed, rupture-prone and ruptured plaques as well as in intravascular thrombosis. They are key players in thrombosis and atherosclerosis. In this study we describe the construction of a radiolabeled single-chain antibody targeting the LIBS-epitope of activated platelets to selectively depict platelet activation and wall-adherent non-occlusive thrombosis in a mouse model with nuclear imaging using in vitro and ex vivo autoradiography as well as small animal SPECT-CT for in vivo analysis.</AbstractText><AbstractText Label="METHODOLOGY/PRINCIPAL FINDINGS" NlmCategory="RESULTS">LIBS as well as an unspecific control single-chain antibody were labeled with (111)Indium ((111)In) via bifunctional DTPA ( = (111)In-LIBS/(111)In-control). Autoradiography after incubation with (111)In-LIBS on activated platelets in vitro (mean 3866 ± 28 DLU/mm(2), 4010 ± 630 DLU/mm(2) and 4520 ± 293 DLU/mm(2)) produced a significantly higher ligand uptake compared to (111)In-control (2101 ± 76 DLU/mm(2), 1181 ± 96 DLU/mm(2) and 1866 ± 246 DLU/mm(2)) indicating a specific binding to activated platelets; P<0.05. Applying these findings to an ex vivo mouse model of carotid artery thrombosis revealed a significant increase in ligand uptake after injection of (111)In-LIBS in the presence of small thrombi compared to the non-injured side, as confirmed by histology (49630 ± 10650 DLU/mm(2) vs. 17390 ± 7470 DLU/mm(2); P<0.05). These findings could also be reproduced in vivo. SPECT-CT analysis of the injured carotid artery with (111)In-LIBS resulted in a significant increase of the target-to-background ratio compared to (111)In-control (1.99 ± 0.36 vs. 1.1 ± 0.24; P < 0.01).</AbstractText><AbstractText Label="CONCLUSIONS/SIGNIFICANCE" NlmCategory="CONCLUSIONS">Nuclear imaging with (111)In-LIBS allows the detection of platelet activation in vitro and ex vivo with high sensitivity. Using SPECT-CT, wall-adherent activated platelets in carotid arteries could be depicted in vivo. These results encourage further studies elucidating the role of activated platelets in plaque pathology and atherosclerosis and might be of interest for further developments towards clinical application.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Heidt</LastName><ForeName>Timo</ForeName><Initials>T</Initials><Affiliation>Department of Cardiology and Angiology, University of Freiburg, Freiburg, Germany. timo.heidt@uniklinik-freiburg.de</Affiliation></Author><Author ValidYN="Y"><LastName>Deininger</LastName><ForeName>Friederike</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Peter</LastName><ForeName>Karlheinz</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Goldschmidt</LastName><ForeName>Jürgen</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Pethe</LastName><ForeName>Annette</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Hagemeyer</LastName><ForeName>Christoph E</ForeName><Initials>CE</Initials></Author><Author ValidYN="Y"><LastName>Neudorfer</LastName><ForeName>Irene</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Zirlik</LastName><ForeName>Andreas</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Weber</LastName><ForeName>Wolfgang A</ForeName><Initials>WA</Initials></Author><Author ValidYN="Y"><LastName>Bode</LastName><ForeName>Christoph</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Meyer</LastName><ForeName>Philipp T</ForeName><Initials>PT</Initials></Author><Author ValidYN="Y"><LastName>Behe</LastName><ForeName>Martin</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>von Zur Mühlen</LastName><ForeName>Constantin</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>03</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS One</MedlineTA><NlmUniqueID>101285081</NlmUniqueID><ISSNLinking>1932-6203</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Epitopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Indium Radioisotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Single-Chain Antibodies</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Circ Cardiovasc Imaging. 2009 Jan;2(1):56-70</RefSource><PMID Version="1">19808565</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Nucl Cardiol. 2009 Sep-Oct;16(5):753-62</RefSource><PMID Version="1">19662466</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Biomaterials. 2010 Aug;31(24):6249-68</RefSource><PMID Version="1">20510444</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Circ Cardiovasc Imaging. 2010 Jul;3(4):464-72</RefSource><PMID Version="1">20442371</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Semin Nucl Med. 2010 Nov;40(6):474-9</RefSource><PMID Version="1">20920637</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Nucl Cardiol. 2000 Jul-Aug;7(4):359-64</RefSource><PMID Version="1">10958278</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Arch Intern Med. 2003 Feb 24;163(4):452-6</RefSource><PMID Version="1">12588204</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Psychiatry. 2004 Jan;9(1):42-54</RefSource><PMID Version="1">14581931</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Pharmacol Exp Ther. 2004 Mar;308(3):1002-11</RefSource><PMID Version="1">14617694</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Nucl Med. 1985 May;26(5):538-45</RefSource><PMID Version="1">3989608</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nat Rev Drug Discov. 2004 Nov;3(11):913-25</RefSource><PMID Version="1">15520814</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Thromb Res. 2005;115(1-2):95-100</RefSource><PMID Version="1">15567459</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Circulation. 2005 Mar 22;111(11):1377-82</RefSource><PMID Version="1">15738354</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>N Engl J Med. 2005 Apr 21;352(16):1685-95</RefSource><PMID Version="1">15843671</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nucl Med Biol. 2005 Oct;32(7):719-25</RefSource><PMID Version="1">16243647</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Clin Invest. 2005 Dec;115(12):3378-84</RefSource><PMID Version="1">16322783</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Circ Res. 2006 Jul 7;99(1):25-33</RefSource><PMID Version="1">16778135</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Circulation. 2006 Oct 3;114(14):1504-11</RefSource><PMID Version="1">17000904</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Circ Cardiovasc Imaging. 2008 Nov;1(3):244-56</RefSource><PMID Version="1">19808549</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Arterioscler Thromb Vasc Biol. 2007 May;27(5):1206-12</RefSource><PMID Version="1">17322097</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Arterioscler Thromb Vasc Biol. 2007 Oct;27(10):2079-93</RefSource><PMID Version="1">17600224</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Thromb Haemost. 2008 Feb;6(2):235-42</RefSource><PMID Version="1">18088342</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Circulation. 2008 Jul 15;118(3):258-67</RefSource><PMID Version="1">18574047</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nat Clin Pract Cardiovasc Med. 2008 Aug;5 Suppl 2:S11-7</RefSource><PMID Version="1">18641600</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Imaging. 2008 Mar-Apr;7(2):59-67</RefSource><PMID Version="1">18706288</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Circulation. 2008 Nov 4;118(19):1953-60</RefSource><PMID Version="1">18936327</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Am Coll Cardiol. 2008 Dec 2;52(23):1847-57</RefSource><PMID Version="1">19038682</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Vasc Res. 2009;46(1):6-14</RefSource><PMID Version="1">18515970</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Stroke. 2009 Apr;40(4):1444-50</RefSource><PMID Version="1">19213951</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Arterioscler Thromb Vasc Biol. 2009 Jul;29(7):983-91</RefSource><PMID Version="1">19213945</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Atherosclerosis. 2010 Mar;209(1):18-27</RefSource><PMID Version="1">19883911</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Autoradiography</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Carotid Artery Thrombosis</DescriptorName><QualifierName MajorTopicYN="Y">physiopathology</QualifierName><QualifierName MajorTopicYN="N">radiography</QualifierName><QualifierName MajorTopicYN="Y">radionuclide imaging</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Epitopes</DescriptorName><QualifierName MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Indium Radioisotopes</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Isotope Labeling</DescriptorName><QualifierName MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Platelet Activation</DescriptorName><QualifierName MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Protein Binding</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Single-Chain Antibodies</DescriptorName><QualifierName MajorTopicYN="Y">diagnostic use</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Tomography, Emission-Computed, Single-Photon</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Tomography, X-Ray Computed</DescriptorName></MeshHeading></MeshHeadingList><OtherID Source="NLM">PMC3068185</OtherID></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year><Month>1</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2011</Year><Month>2</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="epublish"><Year>2011</Year><Month>3</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>4</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>4</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>7</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="doi">10.1371/journal.pone.0018446</ArticleId><ArticleId IdType="pubmed">21479193</ArticleId><ArticleId IdType="pmc">PMC3068185</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=IndiumV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001608 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001608 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= *** parameter Area/wikiCode missing ***
|area= IndiumV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:21479193
|texte= Activated platelets in carotid artery thrombosis in mice can be selectively targeted with a radiolabeled single-chain antibody.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:21479193" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a IndiumV2
| This area was generated with Dilib version V0.5.76. |  |